Involvement of the intestine in type 1 diabetes (master thesis project) at Rigshospitalet

Type 1 diabetes is a massive problem worldwide, and at the Bartholin Institute we are, among other things, investigating how the intestine is involved in the disease. We are looking for a master student to work on one or more of the following projects:

  1. Recently, we identified several dysregulated genes in the intestine from NOD mice, our type 1 diabetes model. These gene variations are potentially important in the pathogenesis of type 1 diabetes and include genes from the innate immune system. The aim is to confirm the gene variations on protein level using western blotting, ELISA, immunohistochemistry/immunofluorescence, and microscopy.
  1. We found a number of gene polymorphisms associated with intestinal permeability, which is increased in type 1 diabetes. We aim to characterise some of these polymorphisms using primarily the Caco-2 intestinal cell line in order to elucidate the function in relation to type 1 diabetes. The project will include techniques like siRNA knockdown/ CRISPR-Cas9, Trans Epithelial Electrical Resistance (TEER) measurements, qPCR, and cell culturing.
  1. Moreover, we discovered that certain lipids are reduced in the intestine of NOD mice and hence could play a role in type 1 diabetes. We need a method to determine how diet-supplemented lipids (those missing in intestine) incorporate into the intestinal mucus of mice. We plan to use click-in chemistry to visualize a choline analog in Caco-2 cells. The choline analog is synthesized into specific lipids, meaning that we can detect their location. When the protocol has been optimized, detection of the choline analog in NOD mice can be performed. This project is trial-and-error based with a high degree of self-determination, since we have not performed the technique before. Methods will include cell culturing, immunohistochemistry, click-in chemistry, microscopy, possibly mice experiments.

The Bartholin Institute holds a type 1 diabetes group and a brain cancer group, and we are currently around 20 people on different levels and with different educational backgrounds. The Institute is located at the Copenhagen Biocenter and is part of the Department of Pathology, Rigshospitalet. We are ambitious, but informal and have a pleasant and friendly environment. Our laboratory is well-equipped with state-of-the-art equipment.

We look for a highly motivated and energetic person. Your master programme is ideally molecular biomedicine, biochemistry, civil engineer, or biology.

For more info, please contact: Group Leader Martin Haupt-Jørgensen martin.haupt-joergensen@regionh.dk or PhD student Mia Mønsted mia.oegaard.moensted@regionh.dk

Check us out at our website https://www.rigshospitalet.dk/english/departments/centre-of-diagnostic-investigation/bartholin-instituttet/Pages/default.aspx

Start date: Soon as possible

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